In order to reduce the energy loss and environmental pollution in the production and construction of asphalt mixture, the rubber hydrocarbon oil was used as the matrix to prepare the modifier at normal temperature. The differential scanning calorimetry, infrared spectrometer and segregation test were used to systematically evaluate the micro performance of normal-temperature modifier and its compatibility with asphalt. On this basis, the normal-temperature modifier was used to modify the matrix asphalt to prepare the normal-temperature modified asphalt. The performance evaluation system and mixture composition design method of the normal-temperature modified asphalt were defined. Based on rutting test, residual stability test and freeze-thaw splitting test, the high-temperature performance and water stability of the normal-temperature modified asphalt mixture were evaluated, and the strength growth law of the mixture was evaluated based on the uniaxial compressive strength test. The results show that: (1)the content of small molecular substances and impurities in the normal-temperature modifier is less, and its compatibility with the matrix asphalt is good; (2)the performance evaluation system of the normal-temperature modified asphalt should not be the performance evaluation system of the road asphalt and the liquid asphalt, but should be proposed in combination with the traffic volume and climate; (3)the normal-temperature modified asphalt mixture should adopt the power function to build the skeleton mineral aggregate gradation, and determine the best ratio of oil and stone with the closest state of mineral aggregate; (4)the high-temperature performance of the normal-temperature modified asphalt mixture needs to be further improved, however, its high-temperature performance meet the requirements of current specifications when it is cured at normal temperature for 15 days; (5)the water stability of the normal-temperature modified asphalt mixture meets the requirements of current specifications; (6)with the increase of curing age, the uniaxial compressive strength of normal-temperature modified asphalt mixture first increases and then decreases, and when the curing age is 14 days, the strength reaches the peak value of 13.23MPa.